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Mouse-spermatid micronucleus assay on the clastogenicity of X-rays and cyclophosphamide

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Mousespermatidmicronucleus (MusSPMN) assay is one of the most relevant test systems for the clastogenicity of environmental pollutants in mammalian germ cells. A “Serial Fixation” of the Xirradiated cell samples (at 12 or 24 h intervals) was used to confirm the sensitive peak for micronuclei (MN) induction and to improve the efficiency of the test. The sensitive peak as revealed by the highest rate of MN in the early stage of spermatid between the treatment and fixation time was found to be 3.5 days. In order to determine the relative effectiveness of the MusSPMN test for chemical clastogenicity detection the Xray and cyclophosphamide (CP) doseresponse studies were carried out for comparison. Xray dosages at 0, 0.5, 1, 2, 3, 4 and 5 Gy (whole body irradiation) induced 0.66, 2.45, 2.22, 4.44, 6.68, 7.00 and 13.34 MN/1000 spermatids respectively. Correlation coefficient and slope values were 0.92 and 0.022 respectively. The CP dose response curve was established when four groups of male mice were treated (ip injections) with 0, 30, 50 and 60 mg/kg of CP, 0.63, 7.33, 8.89 and 15.67 MN/1000 spermatids were induced respectively. The correlation coefficient and slope were 0.68 and 0.12, respectively. Roughly 3.5 ppm CP equals to 3.75 Gy of Xrays. These doserelated MN frequencies induced by clastogens could serve as a potential signal of the possible heritable mutations induced by the lower dosages of the same agent.
Palabras clave: mousespermatid micronucleus, cyclophosphamide, Xrays, dose response
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Sistema de Información Científica Redalyc
Red de Revistas Científicas de América Latina y el Caribe, España y Portugal
Universidad Autónoma del Estado de México
Versión 2.2 beta | 2015
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