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Determination of cadmium and zinc and its relationship to metallothionein levels in swine kidney
Leticia García Rico, Carmen Frasquillo Félix, Refugio Robles Burgueño, Martín Jara Marini;
Revista Internacional de Contaminación Ambiental 2002 18(4)
Resumen
Inglés Español
Cadmium is considered a hazardous environment pollutant. Animals exposed to this metal show cadmium accumulation mainly in their kidneys. Over 60 % of cadmium binds to metallothioneins, i.e., proteins that act as detoxification agents. The purpose of this study was to isolate metallothioneins in swine kidneys and to determine cadmium, zinc, and copper link with this protein. Twenty kidneys were selected and total concentration of cadmium, zinc, and copper were analyzed by atomic absorption spectroscopy and total metallothioneins using the silver saturation method. Metallothioneins were isolated by gel filtration and ion exchange chromatography. AAS-graphite furnace was used to monitor cadmium, zinc, and copper in eluted fractions. Total concentrations of cadmium, zinc, copper, and metallothioneins were 1.20-2.40 µg/g, 20.37-39.82 µg/g, 3.99-14.80 µg/g, and 4.26-101.68 µg/g, respectively. Results show a positive correlation between metallothioneins levels and each of the metals; a Zn-thionein and a Cdthionein were also identified.

Palabras clave: metallothionein, cadmium, zinc, environment indicator
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