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Effects induced by chromium trioxide on root tip chromosomes of Vicia faba

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The cytogenetic effects of chromium trioxide (CrO,) in meristematic root tips of Vkzh faba were evaluated by the analysis of chromosomal alterations in anaphase cells. The CrO, concentrations of 0.001, 0.01, 0.05, 0.10, 0.50, 1 .O, 1.5, 2.0, 2.5, and 3.0% were applied for 1, 2 and 3 hours without recovery. Longer exposures damaged the tissues. This substance induced chromosomal aberrations and also produced injury in the centromere, leading to chromosomes with centromere inactivated and isochromo~nnes as well as alterations of the mitotic spindle causing multipolar anaphases and C-metaphases. No relation in concentration-effect was found because the highest frequencies of abnormal anaphases, total aberrations and multipolar anaphases were noted at 0.01% of CrO,, which decreased at higher concentrations. This behavior was possibly due to the fact that the absorbance of high concentrations of chromium by the roots, impregnated its cell walls therefore avoiding the entrance of more chromium. When colchicine was applied simultaneously to the treatment, the higher CrO, concentration the lower numbeiif C-metaphases in relation to anaphases, thus at low CrO, concentrations the colchicine uptake was higher.
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Sistema de Información Científica Redalyc
Red de Revistas Científicas de América Latina y el Caribe, España y Portugal
Universidad Autónoma del Estado de México
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