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A Model on Agave tequilana Weber for Detection of Damaged DNA from Diseased Cells and Cells Exposed to Glyphosate
Carlos Alvarez Moya, Anne Santerre Lucas, Guillermo Zúñiga González, Eduardo Padilla Camberos, Alfredo Feria Velasco;
Revista Mexicana de Fitopatología 2001 19(1)
Resumen
Inglés Español
The comet assay is a valuable tool to assess for genetic damage induced by chemicals. However, except for one report, this test has been used in animal cells only. In this study, the alkaline comet assay was used in order to detect the presence of lesions in genetic material of Agave tequilana plants. To do this, a simple methodology was designed to obtain the nuclei. Three groups of plants were studied: (a) diseased plants (Agave with wilting) directly taken from crops; (b) healthy plants obtained by somatic embryogenesis; and (c) healthy plants exposed separately to a well known mutagen, namely ethyl methane sulfonate and glyphosate, a herbicide. Glyphosate is extensively applied to agave crops. All plant groups, including the diseased plants, showed a statistically significant difference (p < 0.001) in tail length when compared to healthy plants. The results indicate that the comet assay may be used to diagnose genetic damage in any kind of plant not traditionally used as genotoxicity biomonitors

Palabras clave: Plant genotoxicity test, embryogenesis, maleic hidrazide, ethyl methane sulfonate
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Universidad Autónoma del Estado de México
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