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Inoculum Variables Affecting Pathogenicity of Botrytis cinerea Infection of Kiwifruit
Silvia Bautista Baños, Laura Leticia Barrera Necha, Peter G. Long, Siva Ganesh, Lian-Heng Cheah;
Revista Mexicana de Fitopatología 2001 19(2)
Resumen
Inglés Español
Bautista-Baños, S., Barrera-Necha, L.L., Long, P.G., Ganesh,S., and Cheah, L.H. 2001. Inoculum variables affecting pathogenicity of Botrytis cinerea infection of kiwifruit. Revista Mexicana de Fitopatología 19:161-167.In vitro spore germination of Botrytis cinerea isolates from diseased blueberries, camellia, grapes, kiwifruit andstrawberries grown on malt agar (MA) for 7 or 28 days werecompared. Pathogenicity of grape, kiwifruit and strawberryisolates was tested on kiwifruit at Massey University and atthe New Zealand Institute for Crop and Food Research(Levin). Average spore germination was 74.4% when the suspension concentration was 5.9 x 104, and only 62.8% whenit was 1.5 x 106. The percentage germination on agar did not indicate relative pathogenicity on kiwifruit, and there was no significant difference in disease incidence of fruit inoculatedwith different isolates after 12 weeks of coolstorage at Levin.At Massey University, the grape and kiwifruit isolates causedmore disease than the other isolates. Botrytis storage rot was proportional to inoculum concentration. There was nosignificant effect of culture age. At Massey University; thehighest disease incidence was obtained from inoculum produced on MA, whereas at The Crop and Food ResearchInstitute it was from inoculum produced on autoclavedkiwifruit leaves.

Palabras clave: Actinidia deliciosa, in vitro sporegermination, inoculation.
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Universidad Autónoma del Estado de México
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