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Scions from the sweet potato (Ipomoea batatas) breeding line GA90-16 graft inoculated to I. setosa caused symptoms similar to those caused by the begomovirus, Sweet potato leaf curl virus (SPLCV). Polymerase chain reaction (PCR) using SPLCV-specific primers confirmed the presence of SPLCV. Analyses of partial clones of the AC1 open reading frame sequences indicated that GA90-16 was doubly infected with SPLCV and a related geminivirus, which we propose to designate as Ipomoea leaf curl virus (ILCV). Individual whitefly (Bemisia tabaci biotype B) transmission was conducted to separate these two viruses which could be differentiated by restriction enzyme digestion of PCR (AV1 OR) products utilizing EcoR1. ILCV caused leaf curl symptoms in several Ipomoea species. Unlike SPLCV, ILCV did not cause yellow vein symptoms on I. aquatica and I. cordatotriloba. DNA A of ILCV was cloned and sequenced. The genome organization of the virus was typical of other Old World monopartite begomoviruses. Sequence comparisons showed that ILCV was similar to SPLCV and to another Ipomoea-infecting begomovirus, Ipomoea yellow vein virus (IYVV) from Spain with about 76 and 78% nucleotide sequence identity, respectively. Although the coat protein of ILVC was nearly identical to that of SPLCV and IYVV, the sequence of the common region, and the AC1, AC2, AC3, and AC4 ORFs were sufficiently different to warrant the nomination of a new begomovirus species.

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Universidad Autónoma del Estado de México
Sistema de Información Científica Redalyc ®
Versión 3.0 | 2018
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