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Antifungal Activity of Seed Powders, Extracts, and Secondary Metabolites of Pachyrhizus erosus (L.) Urban (Fabaceae) Against Three Postharvest Fungi
Laura Leticia Barrera Necha, Silvia Bautista Baños, Leticia Bravo Luna, Francisco Javier Leobardo García Suárez, Dagoberto Alavez Solano, Ricardo Reyes Chilpa;
Revista Mexicana de Fitopatología 2004 22(3)
Resumen
Inglés Español
The antifungal effects of seed powders, extracts, and secondary metabolites of Pachyrhizus erosus from Morelos state, Mexico, was investigated using mycelial inhibition bioassays on Colletotrichum gloeosporioides, Fusarium oxysporum, and Rhizopus stolonifer. In general, a dose-effect curve for concentrations 0.5, 2.0, 5.0, and 10 mg/ml was observed on the fungi evaluated. Seed powders had an inhibitory effect on C. gloeosporioides; nevertheless, stimulation effects were observed on F. oxysporum, and R. stolonifer at 0.5, and 5.0 mg/ml, respectively. All extracts, hexane, dicloromethane, and acetone, significantly inhibited the three fungi at 2.0, 5.0, and 10 mg/ml. The greatest fungistatic effects were achieved with dicloromethane extract on R. stolonifer (-64.97%), on F. oxysporum (-37.8%), and C. gloeosporioides (-36.4%). These results suggest that fungicidal compounds might be extracted with this solvent. The dicloromethane extract was subjected to chromatography isolating rotenone (1), erosone (3), paquirrizone (5), dolineone (6), and paquirrizine (4). The isoflavone dehydroneotenone (2) was isolated from the acetone extract. The identity of the components was determined by infrared, ultraviolet, 1H, and 13C nuclear magnetic resonance. These secondary metabolites significantly inhibited the three fungi at 250 µg/ml. The best fungicidal effect was achieved with rotenone on R. stolonifer, with pachyrrizine on F. oxysporum, and dehydroneotenone on C. gloeosporioides.

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Universidad Autónoma del Estado de México
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