Efecto de almidones nativos y modificados sobre el desempeño nutrimental y fisiológico en los juveniles silvestres del mero rojo (Epinephelus morio)

Effect of native and modified starches on nutritional and hysiological performance of wild juveniles of red grouper (Epinephelus morio)

Red grouper (Epinephelus morio) is a new candidate species for aquaculture and represents a commercial species in the Gulf of México; however, overfishing led to a drop of the natural populations. E. morio belongs to Serranidae family and like other groupers, is considered a predator that fed on large variety of prey. In this sense, crabs are the most abundant prey in its stomach content; also shrimps, lobsters, cephalopods, and marine fish can be present (Giménez et al. 2001). This diversity of preys in the daily meáis showed the great flexibility and opportunism at a trophic level where groupers can find an adequate nutrition (Schmitt 1986). Al- though, these studies present E. morio as a colossal carnivore, biochemical information on enzymes involved in digestión or intermediary metabolism enzymes related to feeding habits are scarce (Amirkolaie et al. 2005).

In relation to the nutrition of red grouper a previous work has been done (Silva et al. 2014) focusing on protein sources selection and requirement without consideration for dietary carbohydrates. However, dietary carbohydrate as an alternative to limit protein content in feed (Cowey and Walton 1989) were tested in carnivorous species such as Atlantic salmón Salmo salar (Hemre and Hansen 1998), gilthead seabream Sparus aurata (Metón et al. 2003, Enes et al. 2006, Fernández et al. 2007), snout bream Megalobrama amblycephala (Ren et al. 2015), European seabass Dicentrarchus labrax, (Peres and Oliva-Teles 2002), rainbow trout Oncorhynchus mykiss (Kirchner et al. 2005).

Utilization of carbohydrates depends on feeding habits: omnivorous fish exhibited a higher starch utilization compared to carnivorous fish; glucose or maltose dextrin was utilized faster than higher molecular form, such as starches that contain amylose and amylopectin α-D (l-4)-glycosidic bonds (Wilson 1994). However, the digestión of the different starches depends also on the characteristics related to granule size: cornstarch (5 to 25 µm) can be digested better than potato starch (15 to 100 µm) (Buleon etal. 1998). The amylopectin content is also different: waxy and raw cornstarches with 99 and 73% content, and raw and gelatinized potato starches around 78%. Amylopectin was found more soluble and stable in water solution and do not form starches resistant to digestión (Tharanathan 2002).

Considering the above-mentioned, wild juvenile’s E. morio possess a carbohydrase activity in the pyloric caeca and intestine and will be able to digest dietary starches under controlled conditions. In this sense, to guarantee the effective utilization of glucose in the liver, it needs to be phosphorylated by hexokinase (HK) with low Km (0.1 mM). Glucokinase isoform (HK IV) with high km (5-10 mM) plays a regulator role for glycaemia (Metón et al. 2003, Enes et al. 2006) and the metabolic profile responded as an adaptation to various energy sources. The present study aim to the dietary acclimation of wild juvenile’s E. morio and the effect of native and modified starches from various origins for digestión and intermediary metabolism.

Diets containing starch had no incidence on ingestión rate (p > 0.05) but a positive correlation was observed between final wet weight and ingestión rate (Figure 1). TGC valúes were similar whatever treatment (p > 0.05, Table 2).Diets containing starch had no incidence on ingestión rate (p > 0.05) but a positive correlation was observed between final wet weight and ingestión rate (Figure 1). TGC valúes were similar whatever treatment (p > 0.05, Table 2). Hematocrit (%) was similar for all treatments (p > 0.05, Table 3) as well as plasma glucose level (p > 0.05). Liver glycogen content peaked at 214 ±14 mg g^-1 with waxy cornstarch (p < 0.05); hepatosomatic Índex did not change (p > 0.05). Specific activity of α-amylase gave a mean valué of 2768 U and 448 U for pyloric caeca and intestine respectively and α-glycosidase (mean valué 610 U and 487 for pyloric caeca and intestine respectively did not differ whatever dietary treatment (p > 0.05).

Figure 1
Correlation between final wet weight (g) and ingestion rate (kJ g of fish^-1 of the wild juveniles E. morio. Raw native cornstarch (RCS), waxy cornstarch (WCS), raw potato starch (RPS), gelatinized potato starch (GPS)

Table 2
Zootechnical parameters of wild juveniles of E. morio: initial and final wet weight, survival, thermal growth coefficient (TGC). Raw native cornstarch (RCS), waxy cornstarch (WCS), potato raw starch (PRS), gelatinized potato starch (GPS)

* Different letters in superscript in the same row indicate significant differences. Mean(SE.

Table 3
Physiological parameters of E. morio fed different diets. Raw native cornstarch (RCS), waxy cornstarch (WCS), raw potato starch (RPS), gelatinized potato starch (GPS)

¹ Hematocrit = (viscera weight/body weight) ×100. ²HSI = (liver weight/body weight) ×100. Significant differences in the same row are indicated by different letters (Tukey test, p<0.05). Mean(SE

In liver, metabolic enzymes ofglycolytic path- way reacted according to the nature of starch significantly. HK1, HK2 and GK activities were significantly higher in fish fed raw cornstarch (p < 0.05) but no difference appeared in fish fed waxy cornstarch (p > 0.05). The lowest valúes were ob- served in fish fed gelatinized potato starch; however, no differences were observed in pyruvate kinase ac- tivity (p > 0.05, Table 4). FBPase activity was low in fish fed gelatinized potato starch (p < 0.05), and this was inversely correlated to alanine amino transferase activity, high in this same diet. By last, enzymes belonging to hexose monophosphate shunt presented the highest activity when fish received raw cornstarch (p < 0.05, Table 4).

Table 4
Specific activities (U mg protein^-1) of hepatic intermediary metabolic enzymes in wild juveniles E. morio. Raw native cornstarch (RCS), waxy cornstarch (WCS), raw potato starch (RPS), gelatinized potato starch (GPS)

Mean values ±SE for each parameter (n=10). Significant differences in the same row are indicated by different letters in same row (Tukey test, p<0.05). Mean(SE

This study demonstrated the ability of E. morio to incorpórate dietary glucose from different starches to liver intermediary metabolism, in spite to its carnivorous habit and whose natural diet is constituted mainly on crustaceans, mollusks and fish (Giménez et al. 2001). The results of growth showed no significant differences between diets, estimated through thermal growth coefficient (TGC). That led all opportunity to examine the effect of carbohydrate from various origins on fish metabolism. Carnivorous fish received 20% inclusión level during 93 d with a glycaemia that remained relatively stable, suggesting that E. morio can regúlate glucose homeostasis (Furuichi and Yone 1981) and metabolic aspects through phosphorylation and glycolytic pathway. In similar carnivorous species such as Salmo salar, Hippoglosus hippoglosus, Sparus sarba and D. labrax, GK activity had a fundamental role in the glucose regulation (Leung and Woo 2012, Viegas et al. 2015). Another parameter that could indícate that fish were healthy during the time of experiment was the hematocrit allowing eventually an interaction between nutrients in fish diets in captivity (Wahli 2002). Any change in hematocrit valúes may be a sign of immune-suppression; otherwise stress under hypoxia condition may lead to excessive erythrocytes amount that modify blood formula (Valenzuela et al 2002). In this study, hematocrit valúes fluctuated around 50%, and no significant differences between treatments were found. For red grouper in natural environment, hematocrit valúes have been reported around 40%. It may be that under controlled condition (lower stress) fish fed a diet that covers its nutritional requirements produced a high hematocrit valué (50%).

A high assimilation of native cornstarch and waxy cornstarch in relation to the other dietary treatments was corroborated by TGC valúes; however, no differences were found in α-amylase and α-glycosidase activity between diets. But in the caeca, an increment of digestive activities was observed for all treatments. Wilson (1994) pointed out that a simple carbohydrate was absorbed quickly, producing hyperglycemia, unlike a starch digested slowly. Plasma glucose valúes were stable with all diets whatever starch origin. E. morio as a carnivorous species presented less α-amylase compared to an herbivorous fish (Castillo et al. 2018), when fed corn starch small granules (5-30 µ). That would potentiate a hydrolytic activity, liberating glucose and activating glycolytic routes. The situation is different with large granules ( 100 pm) as in potato that reduced the rate of hydrolysis (Bello-Pérez et al 1996). Native cornstarch with large granules size compared to potato starch possesses a greater contact surface area and produced a lower rate of enzymatic hydrolysis (Tester et al 2004). Cornstarch granules had pores and channels that facilitated a diffusion of α-amylase into the substrate and generate a diffused hydrolysis (Zhang et al 2006). Native cornstarch containing small granules can induce HK-I, HK-II and free G-6P activities; therefore the most obvious result with carbohydrate utilization in E. morio recorded through enzymes activity is a control point to incorpórate plasma glucose. By contrast, potato starch was digested by a process of exo-corrosion beginning in outer membrane to end up inside the granule (Tester and Karkalas 2006). Waxy cornstarch produced the highest weight gain, as a result of a positive energetic balance, and also higher glycogen content in liver. This starch rich in amylopectin (99%) presents ramifications that allow a better access via a (1-4) glycosidic linkages hydrolyzed by α-amylase (Bello-Pérez et al. 1996).

The structure of cornstarch helped absorb granules and utilize starchy fraction as evidenced by HK-I and GK activation. 6PG-DH was more active in juveniles fed native raw cornstarch, which activated energy storage. Glycogen submitted to lipogenesis route lead to ribose-5P via pentose phosphate pathway route. In this sense, GK activity regulates glucose homeostasis in fish, in contrast with other hexokinases having a low affinity for glucose (Km 10 mM). This enzyme is not regulated by G6-P and acted only when plasma glucose concentration was high (saturated HK-I and HK-II). GK increased activity occurred with waxy cornstarch, same diet that produced the highest concentration of glycogen in fish. Additionally, PK acting as a third control point in glycolysis presented an increment of activity with raw cornstarch. In such treatment, fish produced enough G6-P substrate via the routes of energy storage: glycogenesis pathway and PPP that generates reducing compounds for unsaturated fatty acids and ribulose 5-P for tissue build-up. In addition, G6P-DH and 6PG-DH had the highest activity with raw cornstarch (p < 0.05). E. morio could be using G6-P for glycolysis, store energy as glycogen form and increase in weight.

Fish in general have a limited tolerance to glucose; it has been shown that there are different factors that lead fish to have a poor utilization and storage capacity for glucose (Wilson 1994, Hemre et al. 2001). Carnivorous fish can tolérate up to 20% carbohydrate without problems of hyperglycemia. In salmonid (Cho 1992), seabream (Metón et al. 2003), tilapia and yellowtail (Shimeno et al. 1996), pompano (Chuanpeng et al. 2015), fish adapt their metabolism to compénsate with dietary changes. Also, groupers assimilate carbohydrate, allowing energy balance to limit dietary protein utilization (Chen and Tsai 1994). Intermediary metabolism was affected by carbohydrate sources at three levels: glycolysis, gluconeogenesis and pentose shunt via (Méton et al. 1999). Fish fed native cornstarch activated glycolytic enzymes (HK-I, HK-II, GK and PK), driving more energy, to achieve its best weight gain valúes.

Gluconeogenesis is a preferred energy route for fish unlike mammals, using amino acid as substrate to obtain energy precursors: regulatory enzymes (FBPase) responsible to produce glucose de novo, from precursors such as lactate, amino acids, glycerol and fructose, gave an indication whether fish took substrates from protein as energy source. FBPase decrease in fish receiving diets containing raw or waxy cornstarch while glycolysis route being active. In this case, rainbow trout fed raw cornstarch increased enzymes of pentose’s pathway (G6P-DH and 6-PGDH), favoring a source of NADPH (Hemre et al. 2001). As for glycolysis, fish fed raw potato starch produced a low GK activity, suggesting poor glucose utilization.

The results showed that E. morio fed waxy or raw cornstarch presented the highest weight gain. All carbohydrate sources were well digested and blood glucose did not vary so far. There were differences at intermediary metabolic enzymes. Glucokinase activity was measured only in fish fed diets containing carbohydrate. Glycolysis was not influenced by carbohydrate accumulation and liver glycogen increased when E. morio received waxy and native cornstarch. These two diets increased activities of HK-I-II and GK. These results suggest that some starches contribute to regulate carbohydrate metabolism. 6PG-DH increased in fish fed native cornstarch. Therefore, E. morio will cope with a metabolism based on waxy cornstarch containing amylopectin (99%) and will be included in future studies as main carbohydrate source.

The authors wish to thank UNAM (IN 219713-3 project) for the financial support. To Karla Escalante, Jaime Suárez, Elisa Chan-Rivas, Korinthya López, Adriana Paredes for their technical support.