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Performance validation of western blot for anti-HIV antibody detection in blood samples collected on filter paper (DBS)

Abstract: For conducting field studies on human immunodeficiency virus (HIV) serodiagnosis, the use of samples collected on filter paper [dried blood spot (DBS)] is profitable owing to the simplicity in handling and delivering them. Because of these characteristics, th

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Molecular technique for detection and identification of Helicobacter pylori in clinical specimens: a comparison with the classical diagnostic method

Abstract: Helicobacter pylori is a bacterium found in human epithelial cells of the gastrointestinal tract. Its infection is related to different diseases, such as chronic gastritis, peptic ulcers, gastric lymphoma and adenocarcinoma. The infection by H. pylori is pres

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The detection of Bence Jones protein in urine by the heat test helps in diagnosis of multiple myeloma?

Abstract: Multiple myeloma (MM) is a hematologic malignancy caused by the intense indiscriminate proliferation of plasma cells in the bone marrow. In view of clinical suspicion of MM, clinic laboratory tests and imaging tests should be used, among others. Objectives: E

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The frequency of BS-globin haplotypes in the state of Paraná, Brazil, and clinical manifestations of sickle cell anemia

Abstract: Haplotypes in the βS-globin cluster are named according to their geographical origin as Central African Republic (CAR), Benin (BEN), Senegal (SEN), Cameroon (CAM) and Arab-Indian. They are considered to have influence on the diversity of clinical manifestatio

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Evaluation of cytokines in type 1 diabetes patients with and without retinopathy

Abstract: Diabetes mellitus is one of the greatest public health problems in the world; the numerous complications of this disease are the focus of great concern, as they are related to high morbidity and mortality. Among the complications of diabetes, diabetic retinop

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The measurement of serum osmolality and its application to clinical practice and laboratory: literature review

Abstract: Serum osmolality is an essential laboratory parameter to understand several clinical disorders such as electrolyte disturbances, exogenous intoxication and hydration status. Objective: This study aims to update knowledge about the osmolality examination throu

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Tissue immunostaining of growth, pro- and anti-apoptotic biomarkers in myocardial samples from newborns with hypoxic injury

Abstract: Despite the importance of cardiovascular diseases to population health, there is limited knowledge about how neonatal hypoxia, related or not with prematurity, may cause cell injury to cardiomyocytes in the neonatal period and what the consequences to adult l

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Predominant overexpression of CD25/FOXP3, IFN-γ, and suppressive cytokines in high-grade lesion samples infected with human papillomavirus

Abstract: Human papillomavirus (HPV) persistent infection is the leading cause of cervical cancer and its precursor lesions, and the inappropriate immune response is among the factors that contribute to viral persistence. This may be influenced by regulatory T (Treg) c

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Acute lymphoblastic leukemia: primary bone manifestation with hypercalcemia in a child

Abstract: Primary bone manifestation associated with hypercalcemia is an infrequent presentation of acute lymphoblastic leukemia (ALL) in children. An 8-year-old girl was admitted with bone and abdomen pain, vomiting, fever, headache, anemia, elevated serum calcium and

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Applications of electron microscopy in health: the example of epidermolysis bullosa

Abstract: We report the case of a patient with dystrophic epidermolysis bullosa (DEB) diagnosed by transmission electron microscopy (TEM), emphasizing the applications and importance of this technique in the health area. The patient was a male, the only child of young

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